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Abstract
This investigation was undertaken to determine whether well-characterized microorganisms
in pure cultures inoculated into root canals could cause a periapical inflammation
and whether these microorganisms could be isolated from pulpectomized but primarily
noninfected root canals in other parts of the jaw. The experiments were made on fourteen
dogs. In all dogs partial and/or total pulpectomies were performed under aseptic conditions
and on the majority of the premolars in each quadrant of the jaw. The pulpectomized
roots were inoculated with culture broth alone or culture broth with four different
bacterial species (Staphylococcus aureus, Streptocossus sanguis, Pseudomonas aeruginosa, and Bacteroides fragilis). In each dog only one species was inoculated. Thereafter the teeth were sealed with
sterile cotton, fast-setting zinc oxide argard cement, and amalgam fillings. At different
time intervals (from 4 up to 120 days) bacteriologic specimens were taken from the
noninfected teeth by using sampling fluid (VMG I), charcoaled paper points, and transport
medium (VMG III). Bacteriologic samples from the inoculated teeth were taken after
50 and 100 days to test that the inoculated microorganisms were still alive. Cultivation
was carried out both aerobically and anaerobically. Radiographs were taken before
and during the treatments and at the end of the observation period. A histologic examination
was made after the termination of the experiment. The most important findings were
the following. The inoculated microorganisms could be isolated from the majority of
the primarily noninfected roots after 28 to 120 days, which demonstrated that a transport
of microorganisms (anachoresis) had occurred. The roentgenologic examination revealed
that radiolucencies could be observed on most roots. On the teeth where no roentgenologic
changes could be observed no growth was obtained. The morphologic examination demonstrated
a chronic inflammatory reaction on the roots where Pseudomonas aeruginosa and Bacteroides fragilis had been inoculated or could be isolated, while the inflammatory reaction on the
roots with Staphylococcus aureus and Streptococcus sanguis was dominated by polymorphonuclear leukocytes.
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Article info
Footnotes
☆This study was supported by grants from the Faculty of Odontology. Karolinska Institutet.
Identification
Copyright
© 1979 Published by Elsevier Inc.
