The purpose of this study was to evaluate in vitro the antibacterial effect of calcium hydroxide points by using a broth dilution test. Extracts of calcium hydroxide points and a calcium hydroxide paste (Calasept) were tested by using 2 facultative anaerobic bacteria, Streptococcus mutans (ATCC 27352) and Enterococcus faecalis (ATCC 29212). Saline solution was used as a control. Each tested agent was kept in contact with the bacterial species used for the experiment for 5 minutes, 1 hour, 1 day, 2 days, and 5 days. Results showed that Calasept paste was effective in killing the tested bacteria, whereas calcium hydroxide points and saline showed bacterial survival in all experimental periods. It was concluded that calcium hydroxide points extract is not an effective antimicrobial agent against the tested bacteria. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:593-5)
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Accepted: November 4, 2001
Received in revised form: June 22, 2001
Received: April 20, 2001
*The study was registered as nonfunded research project at College of dentistry research center of King Saud University with registration # 1629.
**Reprint requests: Saad Al-Nazhan, BDS, MSD RDS Department, Division of Endodontics College of Dentistry King Saud University P O Box 60169 Riyadh, Saudi Arabia, 11545
© 2002 Mosby, Inc. Published by Elsevier Inc. All rights reserved.