Abstract
Objective. The purpose of this study was to find an effective inactivating agent for chlorhexidine
that would facilitate removal of all residual antimicrobial effect, which may cause
false-negative results during microbiologic culturing. Study Design. L-α-lecithin, Tween 80, and sodium thiosulfate were used in different proportions
to prepare 6 potential inactivating solutions. Nine mL of each inactivating solution
was mixed with 1 mL of 2% chlorhexidine solution. After 5 minutes of equilibration,
0.1 mL of bacterial cell suspension containing 2 × 104 viable cell of Enterococcus faecalis was added to the mixture. At 10 and 60 minutes, 0.1-mL aliquots were withdrawn and
spread over blood agar plates and incubated at 37°C for 72 hours. The number of colony-forming
units on the blood agar plates was determined and recorded. Results. The combination of 3% Tween 80 and 0.3% L-α-lecithin was found to be the most effective
inactivating agent, allowing full recovery of the test organisms in the presence of
chlorhexidine. Conclusion. The present study demonstrated a method to predictably inactivate chlorhexidine.
(Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:617-20)
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Article info
Publication history
Accepted:
December 3,
2001
Received in revised form:
October 16,
2001
Received:
September 24,
2001
Footnotes
*Reprint requests:Ahmad Zamany, DDS University of Connecticut Health Center School of Dental Medicine Department of Endodontology 263 Farmington Ave MC-1715 Farmington, CT 06030 [email protected]
Identification
Copyright
© 2002 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
