Abstract
The practice of pathology is currently undergoing significant change, in large part
due to advances in the analysis of DNA, RNA, and proteins in tissues. These advances
have permitted improved biologic insights into many developmental, inflammatory, metabolic,
infectious, and neoplastic diseases. Moreover, molecular analysis has also led to
improvements in the accuracy of disease diagnosis and classification. It is likely
that, in the future, these methods will increasingly enter into the day-to-day diagnosis
and management of patients. The pathologist will continue to play a fundamental role
in diagnosis and will likely be in a pivotal position to guide the implementation
and interpretation of these tests as they move from the research laboratory into diagnostic
pathology. The purpose of this 2-part series is to provide an overview of the principles
and applications of current molecular biologic and immunologic tests. In Part I, the
biologic fundamentals of DNA, RNA, and proteins and methods that are currently available
or likely to become available to the pathologist in the next several years for their
isolation and analysis in tissue biopsies were discussed. In Part II, advances in
immunohistochemistry and immunofluorescence methods and their application to modern
diagnostic pathology are reviewed. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:56-74)
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Article info
Publication history
Accepted:
July 23,
2001
Received in revised form:
June 11,
2001
Received:
April 16,
2001
Footnotes
*Reprint requests: J. A. Regezi, DDS, MS, S-512, University of California San Francisco, 513 Parnassus Avenue, San Francisco CA 94143-0424, [email protected]
Identification
Copyright
© 2002 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
