Abstract
Objective. The objective was to determine the prevalence of the polymorphisms of the microsomal
epoxide hydrolase (Ephx1 ), glutathione S-transferase μ1 (GSTM1 ), θ1 (GSTT1 ), and π1 (GSTP1 ) genes in patients with oropharyngeal carcinoma. Study Design. Gene polymorphisms in 137 patients with oropharyngeal carcinoma were determined by
polymerase chain reaction and restriction enzyme digestion for xenobiotic metabolizing
enzymes that have been implicated in the carcinogenesis of tobacco-related neoplasias
and compared with a population sample of 99 persons. Results. At Ephx1 (microsomal epoxide hydrolase) codon 113, an overrepresentation of the greater activity
genotype (Tyr/Tyr ) was observed for male ever-smokers alone, both male and female ever-smokers, female
never-smokers alone, and in both male and female never-smokers, compared with a control
population sample. At codon 139, Ephx1 showed no differences. There was an overrepresentation of homozygosity for the GSTT1 (glutathione S-transferase θ1) null allele [but not for the GSTM1 (glutathione S-transferase μ1) null allele] in ever-smokers, when compared with controls.
Polymorphisms at the GSTP1 (glutathione S-transferase π1) locus did not show differences versus controls, although
in the never-smoker cancer sample there was a higher prevalence of the B/B genotype compared with ever-smokers. Conclusion. The Ephx1 codon 113 Tyr/Tyr variant, as well as homozygosity for the GSTT1 null allele, is associated with oropharyngeal carcinogenesis. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:440-5)
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Article info
Publication history
Accepted:
December 14,
2001
Received in revised form:
March 10,
2001
Received:
February 2,
2001
Footnotes
*The present study was supported by grants DE11280 (J.K.H.) and HD0737 (AGA) from the National Institutes of Health and by the Oral Facial Genetics Program at the Indiana University School of Dentistry.
**Reprint requests: James K. Hartsfield, Jr, DMD, PhD, Professor and Chair, Department of Oral Facial Development, DS204, Indiana University School of Dentistry, 1121 Michigan St, Indianapolis, IN 46202-5186,[email protected]
Identification
Copyright
© 2002 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
