Abstract
Objective. The present study was undertaken to determine the frequency and extent of apical
root resorption associated with induced periradicular lesions in mice. Study Design. Bone and root resorption was quantified by using two- and three-dimensional micro-computed
tomography (μ-CT) in the lower first molars of mice subjected to pulp exposure and
infection. Results. μ-CT measurements showed significant apical resorption in exposed and infected teeth,
resulting in an average distal root shortening of 12.7% (P <.001 vs unexposed). These findings were confirmed with three-dimensional reconstituted
images that showed thinning and shortening of the distal root. Tartrate-resistant
acid phosphatase clastic cells were associated with resorption lacunae on the cementum
of root apices, as well as on bone at the periphery of the periradicular lesions.
Brown and Brenn staining showed the presence of bacteria in dentinal tubules adjacent
to resorbed cementum. Conclusions. Apical root resorption is a prominent and consistent finding associated with periradicular
infection in the mouse. This species represents a convenient model for studying the
pathogenesis of inflammatory root resorption in vivo. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:461-8)
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Article info
Publication history
Accepted:
December 4,
2001
Received in revised form:
November 7,
2001
Received:
October 26,
2001
Footnotes
*Reprint requests: P. Stashenko, DMD, PhD, The Forsyth Institute, 140 The Fenway, Boston, MA 02115 [email protected]
**Supported by grants DE-09018, DE-11664, DE-13747, and AG-13333 from the National Institutes of Health and the M. E. Muller Professorship in Bioengineering at Harvard Medical School.
Identification
Copyright
© 2002 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
