Abstract
Objective. Proteases are involved in the invasion and metastasis of carcinoma cells. In vivo,
oral carcinoma cells easily invade the bone tissue and metastasize to the submandibular
and neck lymph nodes. Cathepsin expression has been shown in some neoplastic tissues
and serves as a prognostic indicator. The purpose of this study was to investigate
the relationship between clinicopathohistologic grades and cathepsin expressions in
oral squamous cell carcinoma and to investigate which cathepsin provides prognostic
information for patients with oral carcinoma. Study Design. Immunohistochemical studies were performed on 78 carcinoma samples with monoclonal
antibodies against cathepsins B, H, and L, and a polyclonal antibody against cathepsin
D. Serial sections were stained by hematoxylin-eosin staining and classified by Anneroth's
classification. Cathepsin B, H, L and D activities of blood serum were determined.
Positive results indicative of the presence of cathepsin were investigated to determine
any correlation between a particular cathepsin and histologic malignancy grades, tumor
cell growth, serum cathepsin activities, and clinical factors. Results. Cathepsins B, H, L, and D were positive in every case. Although the labeling indices
for cathepsins B (CB-LI), H (CH-LI), and D (CD-LI) for the cancer cases showed significant
differences from those of controls, cathepsin L (CL-LI) of cancer cases showed no
difference from that of controls (P <.05). A close correlation was found between CD-LI and T categories of TNM classification
(P <.05), and between CD-LI and PCNA-LI (P <.05). Furthermore, a close correlation was found between CD-LI and N categories
in TNM classification (P <.05). Pathologically, a close correlation was found between CB-LI or CD-LI and the
pattern and/or stage of invasion (P <.05). Conclusion. Cathepsin D and B expression were closely correlated with carcinoma invasion and
progression. These proteases may be useful in determining the prognoses of patients
with oral carcinoma. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:446-54)
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Article info
Publication history
Accepted:
December 14,
2001
Received in revised form:
November 12,
2001
Received:
September 4,
2001
Footnotes
*Reprint requests: Goro Kawasaki, DDS, PhD, First Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, 1-7-1 Sakamoto Nagasaki, 852-8588 Japan [email protected]
Identification
Copyright
© 2002 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
