Advertisement

Clinical and histologic evaluation of effects of hyaluronic acid in rat tongue

      Objective

      This study aimed to investigate local and systemic effects of 2 concentrations of hyaluronic acid (HA) in rats.

      Study Design

      Fifty-four female Wistar rats were allocated to 3 treatment groups (25 mg/mL HA, 5.5 mg/mL HA, and 0.9% NaCl) and 3 subgroups of experimental time (7, 60, and 90 days). The rats were evaluated clinically and then killed. The tongue and right kidney were removed and stained by hematoxylin and eosin and picrosirius (tongue only).

      Results

      The clinical changes did not differ significantly between groups (Kruskal-Wallis: P > .05). Histologically there was significantly greater inflammatory response in the groups with HA compared with control (Kruskal-Wallis: P < .001). All kidney samples were normal. The 25 mg/mL HA group had higher collagen formation (analysis of variance mixed models: P = .016).

      Conclusions

      HA causes moderate inflammatory reaction to 7 days and mild to 60 and 90 days, which is compatible with tissue repair, and a slightly increased collagen formation around the material was detected.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      References

        • Lemperle G.
        • Morhenn V.
        • Charrier U.
        Human histology and persistence of various injectable filler substances for soft tissue augmentation.
        Aesthet Plast Surg. 2003; 27: 354-366
        • Fernández-Cossío S.
        • Castaño-Oreja M.T.
        Biocompatibility of two novel dermal fillers: histological evaluation of implants of a hyaluronic acid filler and a polyacrylamide filler.
        Plast Reconstr Surg. 2006; 117: 1789-1796
        • Dastoor S.F.
        • Misch C.E.
        • Wang H.L.
        Dermal fillers for facial soft tissue augmentation.
        J Oral Implantol. 2007; 33: 191-204
        • Vargas K.F.
        • Borghetti R.L.
        • Moure S.P.
        • Salum F.G.
        • Cherubini K.
        • Figueiredo M.A.Z.
        Use of polymethylmethacrylate as permanent filling agent in the jaw, mouth and face regions—implications on dental practice.
        Gerodontology. 2011; (Epub ahead of print)https://doi.org/10.1111/j.1741-358.2011.00479.x
        • Requena L.
        • Requena C.
        • Christensen L.
        • Zimmermann U.S.
        • Kutzner H.
        • Cerroni L.
        • et al.
        Adverse reactions to injectable soft tissue fillers.
        J Am Acad Dermatol. 2011; 64: 1-34
        • Allemann I.B.
        • Baumann L.
        Hyaluronic acid gel (Juvéderm) preparations in the treatment of facial wrinkles and folds.
        Clin Interv Aging. 2008; 3: 629-634
        • Murray C.A.
        • Zloty D.
        • Warshawski L.
        The evolution of soft tissue fillers in clinical practice.
        Dermatol Clin. 2005; 23: 343-363
        • John H.E.
        • Price R.D.
        Perspectives in the selection of hyaluronic acid fillers for facial wrinkles and aging skin.
        Patient Prefer Adherence. 2009; 3: 225-230
        • Vargas A.F.
        • de Amorim N.G.
        • Pitanguy I.
        Complicações tardias dos preechimentos permanentes.
        Rev Bras Cir Plást. 2009; 24: 71-81
        • Manna F.
        • Dentini M.
        • Desideri P.
        • De P.O.
        • Mortilla E.
        • Maras B.
        Comparative chemical evaluation of two commercially available derivatives of hyaluronic acid (Hylaform from rooster combs and Restylane from Streptococcus) used for soft tissue augmentation.
        J Eur Acad Dermatol Venereol. 1999; 13: 183-192
        • Kogan G.
        • Soltés L.
        • Stern R.
        • Gemeiner P.
        Hyaluronic acid: a natural biopolymer with a broad range of biomedical and industrial applications.
        Biotechnol Lett. 2007; 29: 17-25
        • Lemperle G.
        • Rullan P.P.
        • Gauthier-Hazan N.
        Avoiding and treating dermal filler complications.
        Plast Reconstr Surg. 2006; 118: 92S-107S
        • Nestor M.S.
        • Ablon G.R.
        • Stillman M.A.
        The use of a contact cooling device to reduce pain and ecchymosis associated with dermal filler injections.
        J Clin Aesthet Dermatol. 2010; 3: 29-34
        • Patel V.J.
        • Bruck M.C.
        • Katz B.E.
        Hypersensitivity reaction to hyaluronic acid with negative skin testing.
        Plast Reconstr Surg. 2006; 117: 92e-94e
        • Cohen J.L.
        Understanding, avoiding, and managing dermal filler complications.
        Dermatol Surg. 2008; 34: S92-S99
        • Zielke H.
        • Wolber L.
        • Wiest L.
        • Rzany B.
        Risk profiles of different injectable fillers: results from the Injectable Filler Safety study (IFS study).
        Dermatol Surg. 2008; 34: 326-335
        • Vier-Pelisser F.V.
        • Figueiredo M.A.
        • Cherubini K.
        • Braga-Filho A.
        • Figueiredo J.A.
        The effect of head-fractioned teletherapy on pulp tissue.
        Int Endod J. 2007; 40: 859-865
        • Figueiredo J.A.
        • Pesce H.F.
        • Gioso M.A.
        • Figueiredo M.A.
        The histological effects of four endodontic sealers implanted in the oral mucosa: submucous injection versus implant in polyethylene tubes.
        Int Endod J. 2001; 34: 377-385
        • Gomes M.S.
        • Barletta F.B.
        • Della Bona A.
        • Vanni J.R.
        • Pereira CdC
        • Figueiredo J.A.
        Microbial leakage and apical inflammatory response in dog's teeth after root canal filling with different sealers, post space preparation and exposure to the oral environment.
        J Appl Oral Sci. 2007; 15: 429-436
        • Rosa S.C.
        • de Macedo J.L.S.
        Reações adversas a substâncias de preenchimento subcutâneo.
        Rev Soc Bras Cir Plást. 2005; 20: 248-252
        • de Castro A.C.B.
        • Collares M.V.M.
        • Portinho C.P.
        • Dias P.C.
        • Pinto R.A.
        Necrose facial extensa após infiltração com polimetilmetacrilato.
        Rev Bras Otorrinolaringol. 2007; 73: 850
        • Burgess C.M.
        Principles of soft tissue augmentation for the aging face.
        Clin Interv Aging. 2006; 1: 349-355
        • Romagnoli M.
        • Belmontesi M.
        Hyaluronic acid-based fillers: theory and practice.
        Clin Dermatol. 2008; 26: 123-159
        • Zimmermann U.S.
        • Clerici T.J.
        The histological aspects of fillers complications.
        Semin Cutan Med Surg. 2004; 23: 241-250
        • Parada M.B.
        • Michalany N.S.
        • Hassun K.M.
        • Bagatin E.
        • Talarico S.
        A histologic study of adverse effects of different cosmetic skin fillers.
        Skinmed. 2005; 4: 345-349
        • Ghislanzoni M.
        • Bianchi F.
        • Barbareschi M.
        • Alessi E.
        Cutaneous granulomatous reaction to injectable hyaluronic acid gel.
        Br J Dermatol. 2006; 154: 755-758
        • Narins R.S.
        • Brandt F.
        • Leyden J.
        • Lorenc Z.P.
        • Rubin M.
        • Smith S.
        • et al.
        A randomized, double-blind, multicenter comparison of the efficacy and tolerability of Restylane versus Zyplast for the correction of nasolabial folds.
        Dermatol Surg. 2003; 29: 588-595
        • DeLorenzi C.
        • Weinberg M.
        • Solish N.
        • Swift A.
        Multicenter study of the efficacy and safety of subcutaneous nonanimal-stabilized hyaluronic acid in aesthetic facial contouring: interim report.
        Dermatol Surg. 2006; 32: 205-211
        • DeLorenzi C.
        • Weinberg M.
        • Solish N.
        • Swift A.
        The long-term efficacy and safety of a subcutaneously injected large-particle stabilized hyaluronic acid-based gel of nonanimal origin in esthetic facial contouring.
        Dermatol Surg. 2009; 35: 313-321
        • Levy P.M.
        • De Boulle K.
        • Raspaldo H.
        Comparison of injection comfort of a new category of cohesive hyaluronic acid filler with preincorporated lidocaine and a hyaluronic acid filler alone.
        Dermatol Surg. 2009; 35: 332-336
        • Resende V.C.L.
        • Haddad A.
        • Regazzini D.V.
        • Ferreira L.M.
        Avaliação tardia de 1366 casos de preenchimento dérmico com implante de ácido hialurônico em face.
        Rev Bras Cir Plást. 2008; 23: 34
        • Bachmann F.
        • Erdmann R.
        • Hartmann V.
        • Wiest L.
        • Rzany B.
        The spectrum of adverse reactions after treatment with injectable fillers in the glabellar region: results from the Injectable Filler Safety study.
        Dermatol Surg. 2009; 35: 1629-1634
        • Lupton J.R.
        • Alster T.S.
        Cutaneous hypersensitivity reaction to injectable hyaluronic acid gel.
        Dermatol Surg. 2000; 26: 135-137
        • Friedman P.M.
        • Mafong E.A.
        • Kauvar A.N.
        • Geronemus R.G.
        Safety data of injectable nonanimal stabilized hyaluronic acid gel for soft tissue augmentation.
        Dermatol Surg. 2002; 28: 491-494
        • Edwards P.C.
        • Fantasia J.E.
        • Iovino R.
        Foreign body reaction to hyaluronic acid (Restylane): an adverse outcome of lip augmentation.
        J Oral Maxillofac Surg. 2006; 64: 1296-1299
        • Cox S.E.
        Clinical experience with filler complications.
        Dermatol Surg. 2009; 35: 1661-1666
        • Grunebaum L.D.
        • Allemann I.B.
        • Dayan S.
        • Mandy S.
        • Baumann L.
        The risk of alar necrosis associated with dermal filler injection.
        Dermatol Surg. 2009; 35: 1635-1640
        • Toy B.R.
        • Frank P.J.
        Outbreak of Mycobacterium abscessus infection after soft tissue augmentation.
        Dermatol Surg. 2003; 29: 971-973
        • Rousso J.J.
        • Pitman M.J.
        Enterococcus faecalis complicating dermal filler injection: a case of virulent facial abscesses.
        Dermatol Surg. 2010; 36: 1638-1641
        • Lowe N.J.
        • Maxwell C.A.
        • Lowe P.
        • Duick M.G.
        • Shah K.
        Hyaluronic acid skin fillers: adverse reactions and skin testing.
        J Am Acad Dermatol. 2001; 45: 930-933
        • Yoon E.S.
        • Han S.K.
        • Kim W.K.
        Advantages of the presence of living dermal fibroblasts within Restylane for soft tissue augmentation.
        Ann Plast Surg. 2003; 51: 587-592
        • Christensen L.
        Normal and pathologic tissue reactions to soft tissue gel fillers.
        Dermatol Surg. 2007; 33: S168-S175
        • Dadzie O.E.
        • Mahalingam M.
        • Parada M.
        • El H.T.
        • Philips T.
        • Bhawan J.
        Adverse cutaneous reactions to soft tissue fillers-a review of the histological features.
        J Cutan Pathol. 2008; 35: 536-548