Objective
This study aimed to assess NanoBone as a carrier construct for mesenchymal stem cells
(MSCs) and platelet-rich growth factor (PRGF).
Study Design
In the calvarial bone of 8 mature New Zealand White male rabbits, four 8-mm defects
were created. Each defect received one of the following treatments: Group 1, 0.2 mg
Nano-hydroxyapatite (HA) granule + 2 mL culture medium; Group 2, 0.2 mg Nano-HA +
1 mL autologous PRGF + 2 mL acellular culture medium; Group 3, 0.2 mg Nano-HA + 2
mL culture medium containing 100,000 autogenous MSCs; Group 4, 0.2 mg Nano-HA + 2
mL culture medium containing 100,000 autogenous MSCs + 1 mL autologous PRGF.
Result
Histomorphometric analysis at 6 and 12 weeks demonstrated significantly higher bone
formation in group 4 (29.45% and 44.55%, respectively) (P < .05). Bone formation in groups 1, 2, and 3 were as follows: 11.35% and 32.53%,
29.10% and 39.74%, and 25.82% and 39.11%, respectively.
Conclusions
NanoBone with MSCs and PRGF seems to be an effective combination for bone regeneration
in a rabbit calvaria model.
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Article info
Publication history
Published online: May 31, 2012
Accepted:
September 30,
2011
Received in revised form:
August 31,
2011
Received:
June 3,
2011
Footnotes
This study was supported by a grant from the Dental Research Center, Shahid Beheshti Medical University, Tehran, Islamic Republic of Iran.
Identification
Copyright
© 2013 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
