Oral and maxillofacial surgery Online only article| Volume 115, ISSUE 4, e1-e6, April 2013

Comparison of the effects of new folkloric hemostatic agent on peripheral nerve function: an electrophysiologic study in rats


      The aim was to evaluate the effects of a new folkloric medicinal plant extract on peripheral nerve function compared with oxidized regenerated cellulose (OC) and bovine collagen (BC).

      Study Design

      Under ketamine anesthesia a total of 40 male Sprague-Dawley rat right sciatic nerves were identified. Animals were randomly divided into 5 groups: OC, BC, ankaferd blood stopper (ABS), and negative and positive control groups. The recordings of nerve potentials were carried out using an electrophysiologic data acquisition system. After the application of substances, the nerve conduction velocity (NCV) was recorded for immediate (30 min), early (120 min), and delayed (3 wk) effects on nerve function.


      Statistically, differences were not found among the hemostatic agents (OC, BC, and ABS) at baseline and all tested periods (early, immediate, and delayed; P > .05). The positive control group exhibited lower NCV values compared with the other solutions at the 30-minute period (P < .05) as well as the other tested time periods (P > .05). OC exhibited NCV values closer to the positive control group at 120 minutes (P > .05).


      Folkloric medicinal hemostatic agent could be considered as an acceptable hemostatic material without resulting in any serious peripheral nerve function alterations. The possible desirable effects of bovine collagen and undesirable effects of oxidized cellulose on peripheral nerve function should not be overlooked.
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        • Achneck H.E.
        • Sileshi B.
        • Jamiolkowski R.M.
        • Albala D.M.
        • Shapiro M.L.
        • Lawson J.H.
        A comprehensive review of topical hemostatic agents: efficacy and recommendations for use.
        Ann Surg. 2010; 251: 217-228
        • Arx T.V.
        • Jensen S.S.
        • Hänni S.
        • Schenk R.K.
        Haemostatic agents used in periradicular surgery: an experimental study og their efficacy and tissue reactions.
        Int Endod J. 2006; 39: 800-808
        • Alkan A.
        • Inal S.
        • Yildirim M.
        • Baş B.
        • Ağar E.
        The effects of hemostatic agents on peripheral nerve function: an experimental study.
        J Oral Maxillofac Surg. 2007; 65: 630-634
        • Isler S.C.
        • Demircan S.
        • Cakarer S.
        • Cebi Z.
        • Keskin C.
        • Soluk M.
        • Yuzbasioglu E.
        Effects of folk medicinal plant extract Ankaferd blood stopper on early bone healing.
        J Appl Oral Sci. 2010; 18: 409-414
        • Loescher A.R.
        • Robinson P.P.
        The effect of surgical medicaments on peripheral nerve function.
        Br J Oral Maxillofac Surg. 1998; 36: 327-332
        • Dinh P.
        • Hazel A.
        • Palispis W.
        • Suryadevaras S.
        • Gupta R.
        Functional assessment after sciatic nerve injury in a rat model.
        Microsurgery. 2009; 29: 644-649
        • Bain J.R.
        • Mackinnon S.E.
        • Hunter D.A.
        Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat.
        Plast Reconstr Surg. 1989; 83: 129-138
        • Beyazit Y.
        • Kurt M.
        • Kekilli M.
        • Goker H.
        • Haznedaroglu I.C.
        Evaluation of hemostatic effects of Ankaferd as an alternative medicine.
        Altern Med Rev. 2010; 15: 329-336
        • Ercetin S.
        • Haznedaroglu I.C.
        • Kurt M.
        • et al.
        Safety and efficacy of Ankaferd blood stopper in dental surgery.
        UHOD. 2010; 20: 1-5
        • Kurt M.
        • Kacar S.
        • Onal I.K.
        • Akdogan M.
        • Haznedaroglu I.C.
        Ankaferd blood stopper as an effective adjunctive hemostatic agent for the management of life-threatening arterial bleeding of the digestive tract.
        Endoscopy. 2008; 40: E262
        • Kurt M.
        • Oztas E.
        • Kuran S.
        • et al.
        Tandem oral, rectal, and nasal administrations of Ankaferd blood stopper to control profuse bleeding leading to hemodynamic instability.
        Am J Emerg Med. 2009; 27: e1-e2
        • Bilgili H.
        • Kosar A.
        • Kurt M.
        • Onal I.K.
        • Goker H.
        • Captug O.
        • et al.
        Haemostatic efficacy of Ankaferd blood stopper in a swine bleeding model.
        Med Princ Pract. 2009; 18: 165-169
        • Dogan O.F.
        • Ozyurda U.
        • Uymaz O.K.
        • et al.
        New anticoagulant agent for CABG surgery.
        Eur J Clin Invest. 2008; 38: 341-359
        • Oner A.
        • Dogan M.
        • Kaya A.
        • et al.
        New coagulant agent (Ankaferd blood stopper) for open hemorrhages in hemophilia with inhibitor.
        Clin Appl Thromb Hemost. 2010; 16: 705-707
        • Baykul T.
        • Alanoglu E.G.
        • Kocer G.
        Use of Ankaferd blood stopper as a hemostatic agent: a clinical experience.
        J Contemp Dent Pract. 2010; 11: E088-E094
        • Sheela M.L.
        • Ramakrishna M.K.
        • Salimath B.P.
        Angiogenic and proliferative effects of the cytokine VEGF in Ehrlich ascites tumor cells is inhibited by Glycyrrhiza glabra.
        Int Immunopharmacol. 2006; 6: 494-498
        • Yamakoshi J.
        • Kataoka S.
        • Koga T.
        • Ariga T.
        Proanthocyanidinrich extract from grape seeds attenuates the development of aortic atherosclerosis in cholesterol-fed rabbits.
        Atherosclerosis. 1999; 142: 139-149
        • Zhao J.
        • Wang J.
        • Chen Y.
        • Agarwal R.
        Anti-tumor promoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiation-promotion protocol and identification of procyanidin B5-3′-gallate as the most effective antioxidant constituent.
        Carcinogenesis. 1999; 20: 1737-1745
        • Brodbelt A.R.
        • Miles J.B.
        • Foy P.M.
        • Broome J.C.
        Intraspinal oxidised cellulose causing delayed paraplegia after thoracotomy—a report of three cases.
        Ann R Coll Surg Engl. 2002; 84: 97-99
      1. Iwabuchi S, Koike K, Okabe T, Tago S, Murakami T. Iatrogenic paraplegia caused by surgical used for hemostasis during a thoracotomy: report of a case.

        • Nagamatsu M.
        • Podratz J.
        • Windebank A.J.
        • Low P.A.
        Acidity is involved in the development of neuropathy caused by oxidized cellulose.
        J Neurol Sci. 1997; 146: 97-102
        • Katre C.
        • Triantafyllou R.J.
        • Shaw J.
        • Brown S.
        Inferior alveolar nerve damage caused by bone wax in third molar surgery.
        J Oral Maxillofac Surg. 2010; 39: 511-513
        • Wolvius E.B.
        • van der Wal K.G.H.
        Bone wax as a cause of a foreign body granuloma in a cranial defect: a case report.
        Int J Oral Maxillofac Surg. 2003; 32: 656-658
        • Armstrong J.K.
        • Han B.
        • Kuwahara K.
        • Yang Z.
        • Magyar C.E.
        • Dry S.M.
        • et al.
        The effect of three hemostatic agents on early bone healing in an animal model.
        BMC Surg. 2010; 10: 37
        • Kandemir O.
        • Buyukates M.
        • Kandemir N.O.
        • Aktunc E.
        • et al.
        Demonstration of histopathological and immunohistochemical effects of a novel hemostatic agent, ankaferd blood stopper, on vascular tissue in a rat aortic bleeding model.
        J Cardiothorac Surg. 2010; 5: 110
        • Teker A.M.
        • Korkut A.Y.
        • Gedikli O.
        • Kahya V.
        Prospective, controlled clinical trial of ankaferd blood stopper in children undergoing tonsillectomy.
        Int J Pediatr Otorhinolaryngol. 2009; 73: 1472-1475
        • Goker H.
        • Haznedaroglu I.C.
        • Ercetin S.
        • Kirazli S.
        • Akman U.
        • Ozturk Y.
        • Firat H.C.
        Haemostatic actions of the folkloric medicinal plant extract Ankaferd blood stopper.
        J Int Med Res. 2008; 36: 163-170
        • Baumann P.
        • Schumacher H.
        • Hüsing J.
        • Luntz S.
        • Knaebel H.P.
        A randomized, controlled, prospective trial to evaluate the haemostatic effect of Lyostypt versus surgical in arterial bypass anastomosis: “COBBANA” trial.
        Trials. 2009; 10: 91
        • Schonauer C.
        • Tessitore E.
        • Barbagallo G.
        • Albanese V.
        • Moraci A.
        The use of local agents: bone wax, gelatin, collagen, oxidized cellulose.
        Eur Spine J. 2004; 13: 89-96
        • Ryasava J.
        • Homola J.
        • Dostalek J.
        • Krizova P.
        • et al.
        Surface interactions of oxidized cellulose with fibrin(ogen) and platelets.
        Sens Actuators B. 2003; 90: 243-249