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Clinicobiochemical evaluation of turmeric with black pepper and nigella sativa in management of oral submucous fibrosis—a double-blind, randomized preliminary study

Published:August 06, 2016DOI:https://doi.org/10.1016/j.oooo.2016.07.023

      Objective

      To investigate the effectiveness of turmeric with black pepper and nigella sativa in oral submucous fibrosis (OSMF).

      Study Design

      Forty OSMF patients were randomly divided into two groups. The study was performed under a double-blind, randomized design. Group A received turmeric with black pepper and group B received nigella sativa for 3 months. Clinical evaluation was done every 15 days. Patients' serum superoxide dismutase (SOD) levels were assessed before and after treatment and also compared with healthy controls. The response to treatment was analyzed using analysis of variance, paired t test, and unpaired t test.

      Results

      After the treatment, groups A and B showed 3.85 ± 0.22 mm and 3.6 ± 0.07 mm improvement in mouth opening, respectively (P < .01); 87.90% and 78.91% reduction in burning sensation, respectively (P < .01); and +0.62 U/mL and +0.74 U/mL improvement in serum SOD levels, respectively (P < .05). The maximum mouth opening achieved was 8 mm in group A and 7 mm in group B. The mean pretreatment SOD level for controls and patients was 3.61 ± 0.24 U/mL in group A and 2.63 ± 0.18 U/mL in group B.

      Conclusion

      Turmeric with black pepper and nigella sativa improved mouth opening, burning sensation, and SOD levels in the present OSMF study patients; however, further investigations are needed.
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      References

        • Ranganathan K.
        • Devi M.U.
        • Joshua E.
        • Kirankumar K.
        • Saraswathi T.R.
        Oral submucous fibrosis: A case-control study in Chennai, South India.
        J Oral Pathol Med. 2004; 33: 274-277
        • Pindborg J.
        • Mehta F.
        • Gupta P.
        • Daftary D.
        Prevalence of oral submucous fibrosis among 50,915 Indian villagers.
        Br J Cancer. 1968; 22: 646
        • Hazarey V.K.
        • Erlewad D.M.
        • Mundhe K.A.
        • Ughade S.N.
        Oral submucous fibrosis: Study of 1000 cases from central India.
        J Oral Pathol Med. 2007; 36: 12-17
        • Aziz S.R.
        Coming to America: Betel nut and oral submucous fibrosis.
        J Am Dent Assoc. 2010; 141: 423-428
        • Mats J.
        • Palle H.
        Red and white lesions of the oral mucosa.
        in: Glick M. Burket's Oral Medicine. 12th Ed. People's Medical Publishing House, Shelton, CT2015
        • Bagchi M.
        • Balmoori J.
        • Bagchi D.
        • Stohs S.J.
        • Chakrabarti J.
        • Das D.K.
        Role of reactive oxygen species in the development of cytotoxicity with various forms of chewing tobacco and pan masala.
        Toxicology. 2002; 179: 247-255
        • Rajendran R.
        Oral submucous fibrosis: Etiology, pathogenesis, and future research.
        Bull World Health Organ. 1994; 72: 985-996
        • Murti P.R.
        • Bhonsle R.B.
        • Gupta P.C.
        • Daftary D.K.
        • Pindborg J.J.
        • Mehta F.S.
        Etiology of oral submucous fibrosis with special reference to the role of areca nut chewing.
        J Oral Pathol Med. 1995; 24: 145-152
        • Babu S.
        • Bhat R.V.
        • Kumar P.U.
        • et al.
        A comparative clinico-pathological study of oral submucous fibrosis in habitual chewers of pan masala and betelquid.
        J Toxicol Clin Toxicol. 1996; 34: 317-322
        • Rukmini M.S.
        • D'Souza B.
        • D'Souza V.
        Superoxide dismutase and catalase activities and their correlation with malondialdehyde in schizophrenic patients.
        Indian J Clin Biochem. 2004; 19: 114-118
        • Gupta D.
        • Sharma S.C.
        Oral submucous fibrosis: a new treatment regimen.
        J Oral Maxillofac Surg. 1988; 46: 830-833
        • Gupta S.
        • Reddy M.V.R.
        • Harinath B.C.
        Role of oxidative stress and antioxidants in aetiopathogenesis and management of oral submucous fibrosis.
        Indian J Clin Biochem. 2004; 19: 138-141
        • Sharma J.K.
        • Gupta A.K.
        • Mukhija R.D.
        • Nigam P.
        Clinical experience with the use of peripheral vasodilator in oral disorders.
        Int J Oral Maxillofac Surg. 1987; 16: 695-699
        • Haque M.F.
        • Meghji S.
        • Nazir R.
        • Harris M.
        Interferon gamma (IFN-gamma) may reverse oral submucous fibrosis.
        J Oral Pathol Med. 2001; 30: 12-21
        • Hastak K.
        • Lubri N.
        • Jakhi S.D.
        • et al.
        Effect of turmeric oil and turmeric oleoresin on cytogenetic damage in patients suffering from oral submucous fibrosis.
        Cancer Lett. 1997; 116: 265-269
        • Kumar A.
        • Bagewadi A.
        • Keluskar V.
        • Singh M.
        Efficacy of lycopene in the management of oral submucous fibrosis.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007; 103: 207-213
        • Lai D.R.
        • Chen H.R.
        • Lin L.M.
        • Huang Y.L.
        • Tsai C.C.
        Clinical evaluation of different treatment methods for oral submucous fibrosis. A 10-year experience with 150 cases.
        J Oral Pathol Med. 1995; 24: 402-406
        • Lin H.-J.
        • Lin J.-C.
        Treatment of oral submucous fibrosis by collagenase: Effects on oral opening and eating function.
        Oral Dis. 2007; 13: 407-413
        • Mehrotra R.
        • Singh H.P.
        • Gupta S.C.
        • Singh M.
        • Jain S.
        Pentoxifylline therapy in the management of oral submucous fibrosis.
        Asian Pac J Cancer Prev. 2011; 12: 971-974
        • Tai Y.S.
        • Liu B.Y.
        • Wang J.T.
        • Sun A.
        • Kwan H.W.
        • Chiang C.P.
        Oral administration of milk from cows immunized with human intestinal bacteria leads to significant improvements of symptoms and signs in patients with oral submucous fibrosis.
        J Oral Pathol Med. 2001; 30: 618-625
        • Cox S.
        • Zoellner H.
        Physiotherapeutic treatment improves oral opening in oral submucous fibrosis.
        J Oral Pathol Med. 2009; 38: 220-226
        • Huang J.-J.
        • Wallace C.
        • Lin J.-Y.
        • et al.
        Two small flaps from one anterolateral thigh donor site for bilateral buccal mucosa reconstruction after release of submucous fibrosis and/or contracture.
        J Plast Reconstr Aesthet Surg. 2010; 63: 440-445
        • Mehrotra D.
        • Pradhan R.
        • Gupta S.
        Retrospective comparison of surgical treatment modalities in 100 patients with oral submucous fibrosis.
        Oral Surg Oral Med Oral Path Oral Radiol Endod. 2009; 107: e1-e10
        • Tsao C.-K.
        • Wei F.-C.
        • Chang Y.-M.
        • et al.
        Reconstruction of the buccal mucosa following release for submucous fibrosis using two radial forearm flaps from a single donor site.
        J Plast Reconstr Aesthet Surg. 2010; 63: 1117-1123
        • Baum L.
        • Ng A.
        Curcumin interaction with copper and iron suggests one possible mechanism of action in Alzheimer's disease animal models.
        J Alzheimers Dis. 2004; 6 (discussion 443-449): 367-377
        • Chattopadhyay I.
        • Biswas K.
        • Bandyopadhyay U.
        • Banerjee R.K.
        Turmeric and curcumin: Biological actions and medicinal applications.
        Curr Sci. 2004; 87: 44-53
        • Aggarwal B.B.
        • Kumar A.
        • Aggarwal M.S.
        • Shishodia S.
        Curcumin derived from turmeric (Curcuma longa): A spice for all seasons.
        in: Bagchi D. Preuss H. Phytopharmaceuticals in Cancer Chemoprevention. CRC Press, Boca Raton, FL2005
        • Sa G.
        • Das T.
        • Banerjee S.
        • Chakraborty J.
        Curcumin: From exotic spice to modern anticancer drug.
        Al Ameen J Med Sci. 2010; 3: 21-37
        • Subramanian M.
        • Subramanian M.
        • Rao M.N.
        • Devasagayam T.P.
        • Singh B.B.
        Diminution of singlet oxygen-induced DNA damage by curcumin and related antioxidants.
        Mutat Res. 1994; 311: 249-255
        • Punithavathi D.
        • Venkatesan N.
        • Babu M.
        Curcumin inhibition of bleomycin-induced pulmonary fibrosis in rats.
        Br J Pharmacol. 2000; 131: 169-172
        • Ahmad A.
        • Husain A.
        • Mujeeb M.
        • et al.
        A review on therapeutic potential of Nigella sativa: A miracle herb.
        Asian Pac J Trop Biomed. 2013; 3: 337-352
        • Chehl N.
        • Chipitsyna G.
        • Gong Q.
        • Yeo C.J.
        • Arafat H.A.
        Anti-inflammatory effects of the Nigella sativa seed extract, thymoquinone, in pancreatic cancer cells.
        HPB. 2009; 11: 373-381
        • Majdalawieh A.F.
        • Hmaidan R.
        • Carr R.I.
        Nigella sativa modulates splenocyte proliferation, Th1/Th2 cytokine profile, macrophage function and NK anti-tumor activity.
        J Ethnopharmacol. 2010; 131: 268-275
        • Nikakhlagh S.
        • Rahim F.
        • Aryani F.H.
        • Syahpoush A.
        • Brougerdnya M.G.
        • Saki N.
        Herbal treatment of allergic rhinitis: The use of Nigella sativa.
        Am J Otolaryngol. 2011; 32: 402-407
        • Raval B.P.
        • Shah T.G.
        • Patel J.D.
        • Patel B.A.
        • Patel R.K.
        Potent anticancer activity of nigella sativa seeds.
        Arch Applied Sci Res. 2010; 2: 52-56
        • Haq A.
        • Abdullatif M.
        • Lobo P.I.
        • Khabar K.S.
        • Sheth K.V.
        • al-Sedairy S.T.
        Nigella sativa: Effect on human lymphocytes and polymorphonuclear leukocyte phagocytic activity.
        Immunopharmacology. 1995; 30: 147-155
        • Ait Mbarek L.
        • Ait Mouse H.
        • Elabbadi N.
        • et al.
        Anti-tumor properties of blackseed (Nigella sativa L.) extracts.
        Braz J Med Biol Res. 2007; 40: 839-847
        • Meddah B.
        • Ducroc R.
        • El Abbes Faouzi M.
        • et al.
        Nigella sativa inhibits intestinal glucose absorption and improves glucose tolerance in rats.
        J Ethnopharmacol. 2009; 121: 419-424
        • Türkdoğan M.K.
        • Ağaoğlu Z.
        • Yener Z.
        • Sekeroğlu R.
        • Akkan H.A.
        • Avci M.E.
        The role of antioxidant vitamins (C and E), selenium and Nigella sativa in the prevention of liver fibrosis and cirrhosis in rabbits: New hopes.
        Dtsch Tierarztl Wochenschr. 2001; 108: 71-73
        • Shoba G.
        • Joy D.
        • Joseph T.
        • Majeed M.
        • Rajendran R.
        • Srinivas P.S.
        Influence of piperine on the pharmacokinetics of curcumin in animals and human volunteers.
        Planta Med. 1998; 64: 353-356
        • Sudarshan R.
        • Annigeri R.G.
        • Sree Vijayabala G.
        Aloe vera in the treatment for oral submucous fibrosis: a preliminary study.
        J Oral Pathol Med. 2012; 41: 755-761
        • Alam S.
        • Ali I.
        • Giri K.Y.
        • et al.
        Efficacy of aloe vera gel as an adjuvant treatment of oral submucous fibrosis.
        Oral Surg Oral Med Oral Pathol Oral Radiol. 2013; 116: 717-724
        • Mathur R.M.
        • Jha T.
        Normal oral flexibility: A guideline for SMF cases.
        J Ind Dent Assoc. 1993; 64: 139-143
        • Marklund S.
        • Marklund G.
        Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase.
        Eur J Biochem. 1974; 47: 469-474
        • Cox S.C.
        • Walker D.M.
        Oral submucous fibrosis: A review.
        Aust Dent J. 1996; 41: 294-299
        • Chiu C.-J.
        • Chang M.-L.
        • Chiang C.-P.
        • Hahn L.-J.
        • Hsieh L.-L.
        • Chen C.-J.
        Interaction of collagen-related genes and susceptibility to betel quid-induced oral submucous fibrosis.
        Cancer Epidemiol Biomarkers Prev. 2002; 11: 646-653
        • Manvi F.
        • Nanjawade B.
        • Shing S.
        Pharmacological screening of combined extract of annona squamosa and nigella sativa.
        IJPBS. 2011; 2: 520-529
        • Bourgou S.
        • Pichette A.
        • Marzouk B.
        • Legault J.
        Antioxidant, anti-inflammatory, anticancer and antibacterial activities of extracts from nigella sativa (black cumin) plant parts.
        J Food Biochem. 2012; 36: 539-546
        • Srivastava A.
        • Agarwal R.
        • Chaturvedi T.P.
        • Chandra A.
        • Singh O.P.
        Clinical evaluation of the role of tulsi and turmeric in the management of oral submucous fibrosis: A pilot, prospective observational study.
        J Ayurveda Integr Med. 2015; 6: 45-49
        • Manoharan S.
        • Balakrishnan S.
        • Menon V.P.
        • Alias L.M.
        • Reena A.R.
        Chemopreventive efficacy of curcumin and piperine during 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis.
        Singapore Med J. 2009; 50: 139-146
        • Bamosa A.O.
        • Kaatabi H.
        • Lebdaa F.M.
        • Elq A.-M.A.
        • Al-Sultanb A.
        Effect of Nigella sativa seeds on the glycemic control of patients with type 2 diabetes mellitus.
        Indian J Physiol Pharmacol. 2010; 54: 344-354
        • Karemore T.V.
        • Motwani M.
        Evaluation of the effect of newer antioxidant lycopene in the treatment of oral submucous fibrosis.
        Indian J Dent Res. 2012; 23: 524-528
        • Jirge V.
        • Shashikanth M.C.
        • Ali I.M.
        • Anshumalee N.
        Levamisole and antioxidants in the management of oral submucous fibrosis: A comparative study.
        J Indian Acad Oral Med Radiol. 2008; 20: 135-140
        • Rajendran R.
        • Rani V.
        • Shaikh S.
        Pentoxifylline therapy: A new adjunct in the treatment of oral submucous fibrosis.
        Indian J Dent Res. 2006; 17: 190-198
        • Angadi P.V.
        • Rekha K.P.
        Oral submucous fibrosis: A clinicopathologic review of 205 cases in Indians.
        J Oral Maxillofac Surg. 2011; 15: 15-19
        • Shah N.
        • Sharma P.P.
        Role of chewing and smoking habits in the etiology of oral submucous fibrosis (OSF): A case-control study.
        J Oral Pathol Med. 1998; 27: 475-479
        • Metkari S.
        • Tupkari J.
        • Barpande S.
        An estimation of serum malondialdehyde, superoxide dismutase and vitamin A in oral submucous fibrosis and its clinicopathologic correlation.
        J Oral Maxillofac Pathol. 2007; 11: 23-27
        • Rai B.
        • Kaur J.
        • Jacobs R.
        • Singh J.
        Possible action mechanism for curcumin in pre-cancerous lesions based on serum and salivary markers of oxidative stress.
        J Oral Sci. 2010; 52: 251-256