Objectives
Tissue hypoxia in oral submucous fibrosis (OSMF) induces hypoxia-inducible factor
(HIF)-1 α and vascular endothelial growth factor (VEGF), causing angiogenesis. Single
nucleotide polymorphisms (SNPs) may predict susceptibility to environmental carcinogens
and to development of OSMF, as well as its severity and malignant transformation.
This study aimed to determine the serologic levels and frequencies of SNPs of HIF-1
α and VEGF in OSMF.
Study Design
In this prospective pilot study, the frequencies of SNPs of HIF-1 α (C1772 T, G1790
A); VEGF-A 936 C/T; and VEGF-C (rs7664413, rs1485766) in patients with OSMF or oral
squamous cell carcinoma (OSCC) and in healthy controls were determined by using polymerase
chain reaction (PCR) (n = 100 each), and serologic levels were determined by using
enzyme-linked immunosorbent assay (ELISA (n = 50 each), in a North Indian population.
Results
Heterozygous forms of HIF-1 α C1772 T (CT: odds ratio [OR] 5.0; 95% confidence interval [CI] 2.24–11.16; P < .001); HIF-1 α G1790 A (GA: OR 2.8; 95% CI 1.62–5.16; P < .001); and VEGF-C rs1485766 (AC: OR 2.18; 95% CI 1.19–3.99; P < .05) were associated with OSMF. The mean serologic levels of HIF-1 α, VEGF-A, and
VEGF-C were significantly raised in patients with OSMF compared with healthy controls
(P < .001).
Conclusions
The SNPs of HIF-1 α, VEGF-A, and VEGF-C and their serologic levels can act as prognostic
biomarkers and aid in the development of specialized anti-HIF-1 α or anti-VEGF drugs
for the management and prevention of OSCC in patients with OSMF.
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Article info
Publication history
Published online: September 25, 2020
Accepted:
August 2,
2020
Received in revised form:
June 4,
2020
Received:
May 8,
2020
Identification
Copyright
© 2020 Elsevier Inc. All rights reserved.
