If you don't remember your password, you can reset it by entering your email address and clicking the Reset Password button. You will then receive an email that contains a secure link for resetting your password
If the address matches a valid account an email will be sent to __email__ with instructions for resetting your password
Recently, fibulin-1 (FBLN1) has been shown to be downregulated in various cancers via promoter hypermethylation.
Our study aimed to determine the expression and methylation status of FBLN1 in tongue
squamous cell carcinoma (TSCC) tissues and cells.
Methylation-specific PCR was implemented to detect the methylation status of the FBLN1
gene in TSCC tissues and Western blot analysis was used to detect the expression of
FBLN1 protein. The human TSCC cell lines, CAL27 and SCC9, were cultured in vitro and treated with 5-aza-deoxycytidine (5-Aza-dC). CCK-8, colony formation, and Transwell
assays were performed to test TSCC cell proliferation, migration, and invasion following
5-Aza-dC treatment or overexpression of FBLN1, which was further verified in in vivo experiments.
FBLN1 was hypermethylated and the protein expression was reduced in TSCC tissues.
After human TSCC cell lines (CAL27 and SCC9) were treated with 5-Aza-dC or overexpressed
FBLN1, FBLN1 expression was upregulated and the TSCC cell proliferation, migration,
and invasion abilities were suppressed. In vivo experiments further showed that demethylation or overexpression of FBLN1 slowed tumor
growth in nude mice.
This study demonstrated that 5-Aza-dC treatment or overexpression of FBLN1 inhibited the growth of human TSCC.