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UCHL1 promotes proliferation and metastasis in head and neck squamous cell carcinoma and could be a potential therapeutic target

  • Author Footnotes
    ⁎ Co-first authors.
    Dahe Zhang
    Footnotes
    ⁎ Co-first authors.
    Affiliations
    Department of Oral & Maxillofacial - Head & Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Shanghai, China

    College of Stomatology, Shanghai Jiao Tong University, Shanghai, Shanghai, China

    National Center for Stomatology, Shanghai, Shanghai, China

    National Clinical Research Center for Oral Diseases, Shanghai, Shanghai, China

    Shanghai Key Laboratory of Stomatology, Shanghai, Shanghai, China
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  • Author Footnotes
    ⁎ Co-first authors.
    You Fu
    Footnotes
    ⁎ Co-first authors.
    Affiliations
    Department of Oral & Maxillofacial - Head & Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Shanghai, China

    College of Stomatology, Shanghai Jiao Tong University, Shanghai, Shanghai, China

    National Center for Stomatology, Shanghai, Shanghai, China

    National Clinical Research Center for Oral Diseases, Shanghai, Shanghai, China

    Shanghai Key Laboratory of Stomatology, Shanghai, Shanghai, China
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  • Guocai Tian
    Affiliations
    Department of Oral & Maxillofacial - Head & Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Shanghai, China

    College of Stomatology, Shanghai Jiao Tong University, Shanghai, Shanghai, China

    National Center for Stomatology, Shanghai, Shanghai, China

    National Clinical Research Center for Oral Diseases, Shanghai, Shanghai, China

    Shanghai Key Laboratory of Stomatology, Shanghai, Shanghai, China
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  • Jiayi Li
    Affiliations
    Department of Oral & Maxillofacial - Head & Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Shanghai, China

    College of Stomatology, Shanghai Jiao Tong University, Shanghai, Shanghai, China

    National Center for Stomatology, Shanghai, Shanghai, China

    National Clinical Research Center for Oral Diseases, Shanghai, Shanghai, China

    Shanghai Key Laboratory of Stomatology, Shanghai, Shanghai, China
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  • Dihua Shang
    Affiliations
    Department of Oral & Maxillofacial - Head & Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Shanghai, China

    College of Stomatology, Shanghai Jiao Tong University, Shanghai, Shanghai, China

    National Center for Stomatology, Shanghai, Shanghai, China

    National Clinical Research Center for Oral Diseases, Shanghai, Shanghai, China

    Shanghai Key Laboratory of Stomatology, Shanghai, Shanghai, China
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  • Shanghui Zhou
    Correspondence
    Corresponding Author: Shanghui Zhou, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639, Zhi-zao-ju Road, Shanghai 200011, P.R. China.
    Affiliations
    Department of Oral & Maxillofacial - Head & Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Shanghai, China

    College of Stomatology, Shanghai Jiao Tong University, Shanghai, Shanghai, China

    National Center for Stomatology, Shanghai, Shanghai, China

    National Clinical Research Center for Oral Diseases, Shanghai, Shanghai, China

    Shanghai Key Laboratory of Stomatology, Shanghai, Shanghai, China
    Search for articles by this author
  • Author Footnotes
    ⁎ Co-first authors.
Published:November 17, 2021DOI:https://doi.org/10.1016/j.oooo.2021.11.004

      Abstract

      Objective

      To research the physiological roles of ubiquitin carboxyl-terminal esterase L1 (UCHL1) in head and neck squamous cell carcinoma (HNSCC).

      Study Design

      Ten HNSCC samples and matched normal oral mucosal tissues were collected. UCHL1 expression of these tissues was detected by the immunohistochemical staining and real-time quantitative polymerase chain reaction. The human HNSCC cell line HN6 UCHL1 knockout (UCHL1 KO) cell line was constructed by CRISPR/CAS9 gene editing and verified by western blotting. Wound healing assay, cell proliferation assay, cell invasion assay, and flow cytometric analysis of the cell cycle and apoptosis were applied to research the role of UCHL1 in HNSCC. Besides, a RNAseq gene expression data set and HNSCC patient survival data from The Cancer Genome Atlas were analyzed.

      Results

      UCHL1 was highly expressed in HNSCC tissues compared to normal oral mucosal tissues (P=0.032). A decreased proliferation (P<0.0001), migration (P<0.0001), and invasion (P=0.0049) ability of HN6 cells was exhibited after knockout of UCHL1. However, HN6 UCHL1 KO cells showed no significant differences in the cell cycle or apoptosis. The progression, nodal metastasis status and stage of HNSCC had a positive correlation with the expression of UCHL1.

      Conclusions

      UCHL1 plays an important role in HNSCC and we consider that targeting UCHL1 may be a feasible therapeutic strategy for HNSCC.

      Keywords

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