The aim of this study was to investigate and compare the immunohistochemical expression of connexin 43 (Cx43) in tooth germs (TGs), ameloblastic fibromas (AFs), ameloblastic fibro-odontomas (AFOs), and conventional ameloblastomas (AMs).
Nine TGs, 12 AFs, 12 AFOs, and 27 AMs were evaluated for Cx43 expression by immunohistochemistry.
Most of the TGs expressed Cx43 in the mesenchyme (77.6%) and in the late stages of odontogenesis. Cx43 was more highly expressed (P < .05) in the mesenchymal layer of all groups than in the epithelial layer except for the AFOs. When comparing the expression of Cx43 in the different layers of the analyzed groups, statistically significant differences were observed between AFO vs AM (*P = .0158) in the epithelial layer and between AF vs AFO (P** = .0046) in the mesenchymal layer.
The results obtained in this study showed that Cx43 is a protein with important expression in the mesenchymal layer of the embryonic and odontogenic tissues studied. It could be speculated that Cx43 participates in mineralization events based on the relationship of the expression of this protein between the epithelial and mesenchymal layers of odontogenic tissues.
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- Immunohistochemical expression of neural tissue markers (neuron-specific enolase, glial fibrillary acidic protein, S100 protein) in ameloblastic fibrodentinoma: a comparative study with ameloblastic fibroma.Pathol Int. 2000; 50: 610-615
- New diagnostic molecular markers and biomarkers in odontogenic tumors.Mol Biol Rep. 2021; 48: 3617-3628
- Ameloblastoma: current etiopathological concepts and management.Oral Dis. 2018; 24: 307-316
- Ameloblastomas of the mandible and maxilla.Ear Nose Throat J. 2018; 97: E26-E32
- Clinicopathological study of 100 Nigerian cases of ameloblastoma.Niger Postgrad Med J. 2008; 15: 1-5
- Microgenomics of ameloblastoma.J Dent Res. 2011; 90: 463-469
- Update from the 5th Edition of the World Health Organization Classification of Head and Neck Tumors: odontogenic and maxillofacial bone tumours.Head Neck Pathol. 2022; 16: 63-75
- Connexins: mechanisms regulating protein levels and intercellular communication.FEBS Lett. 2014; 588: 1212-1220
- Transduction of cell survival signals by Connexin-43 hemichannels.J Biol Chem. 2002; 277: 8648-8657
- Plasma membrane channels formed by connexins: their regulation and functions.Physiol Rev. 2003; 83: 1359-1400
- Implications and challenges of connexin connections to cancer.Nat Rev Cancer. 2010; 10: 435-441
- Connexins: a myriad of functions extending beyond assembly of gap junction channels.Cell Commun Signal. 2009; 7: 4
- Connexin43 deficiency causes delayed ossification, craniofacial abnormalities, and osteoblast dysfunction.J Cell Biol. 2000; 151: 931-944
- In vivo and in vitro expression of Connexin 43 in human teeth.Connect Tissue Res. 2002; 43: 232-237
- The Importance of Connexin 43 in Enamel Development and Mineralization.Front Physiol. 2018; 9: 750
- Expression of connexin 43 and ZO-1 in differentiating ameloblasts and odontoblasts from rat molar tooth germs.Histochem Cell Biol. 2003; 119: 21-26
- Epithelial - mesenchymal interactions in tooth development and the significant role of growth factors and genes with emphasis on mesenchyme—a review.J Clin Diagn Res. 2016; 10: ZE05-ZE09
- Decreased levels of Cx43 gap junctions result in ameloblast dysregulation and enamel hypoplasia in Gja1Jrt/+ mice.J Cell Physiol. 2010; 223: 601-609
- Gene expression profiles in dental follicles from patients with impacted canines.Odontology. 2018; 106: 351-359
- Mixed odontogenic tumors: a review of the clinicopathological and molecular features and changes in the WHO classification.World J Clin Oncol. 2021; 12: 1227-1243
Published online: January 31, 2023
Accepted: January 25, 2023
Received in revised form: October 28, 2022
Received: September 14, 2022
Publication stageIn Press Journal Pre-Proof
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